The mod­ern day pros­e­cu­tion for the unlaw­ful pos­ses­sion of mar­i­juana is based upon a three test régime involv­ing micro­scopic mor­pho­log­i­cal exam­i­na­tion, mod­i­fied Duquenois-Levine col­ori­met­ric test­ing, and Thin Layer Chro­matog­ra­phy. Each of these three tests are non-specific for THC which is the phar­ma­co­dy­nam­i­cally active ingre­di­ent which makes mar­i­juana ille­gal. The ques­tion becomes is this three test bat­tery col­lec­tively con­clu­sively spe­cific to arrive at a valid con­clu­sion that the unknown seized and tested is in fact mar­i­juana (con­tains THC) and there is no pos­si­bil­ity of a false positive?

In this series of posts we are going to exam­ine this seemly sim­ple question:

  1. What is the goal and the pur­pose of test­ing of unknowns gen­er­ally? How do we best design a test for marijuana?
  2. How is most mar­i­juana test­ing con­ducted in the United States?
  3. What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?
  4. What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?
  5. What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?
  6. Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?
  7. Is there a bet­ter way to test for marijuana?

Part 6: Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?

The gov­ern­ment would choose to argue that the com­bi­na­tion of these three tests results in a valid con­clu­sion that this tested unknown is mar­i­juana (con­tains THC). All  three roads leads, so they say, lead to THC. The more sophis­ti­cated ver­sion of this argu­ment is the Venn dia­gram. Per­haps some­thing like the below:

Venn diagram that the prosecution argues
Venn dia­gram that the pros­e­cu­tion argues

This is sim­ply not proven or sup­ported by the pub­lished empir­i­cal research. Even if it were true there is no evi­dence how wide or big that “D” area is. It could be very wide and large con­tain­ing many com­pounds such as this below exam­ple below where the result of this type of test­ing would be a pos­i­tive for O, T, H, P, M, A, B, X, K, Y and not for B alone:

What could be in there? We don't know.
What could it be? We don’t know.

There is no evi­dence that these dis­tinct tests ever intercept.

Maybe these three tests have nothing in common
Maybe these three tests have noth­ing in common

While in ear­lier posts, we have exam­ined the pro­pri­ety and suit­abil­ity of these three sep­a­rate and dis­tinct tests in the iden­ti­fi­ca­tion of THC, we can­not prop­erly judge them in iso­la­tion. We must remem­ber and in fair­ness this is a 3 test process and technique.

There are no mean­ing­ful or robust stud­ies pub­lished that truly val­i­dates this three test pro­ce­dure as result­ing in a spe­cific qual­i­ta­tive mea­sure for THC.

What com­pounds the issue of the pos­si­bil­ity of error is that as the ana­lyst is not using the same sam­ple through­out each of these 3 tests. It is in fact, three dif­fer­ent sam­ples of the orig­i­nal unknown. The ana­lyst pre­sumes that the unknown is homoge­nous. Fur­ther assumed is that the sam­pling and the sam­ple selec­tion of the unknown con­ducted by the ana­lyst result in iden­ti­cal homo­ge­neous sam­ples. These are not jus­ti­fied sci­en­tific assump­tions (An assump­tion is not drawn from evi­dence; it is a hypoth­e­sis {my assump­tion can be tested by look­ing at the dic­tio­nary}. A pre­sump­tion implies a basis in evi­dence {the legal pre­sump­tion of innocence})

Each of these tests are wholly destruc­tive in nature by their very process.

  • Just because the sought after fea­tures of the micro­scopic mor­pho­log­i­cal exam­i­na­tion were present in the first sam­ple doesn’t nec­es­sar­ily mean that that this sam­ple will con­tain the sought after fea­tures in the mod­i­fied Duquenois-Levine and the TLC exam­i­na­tion because they are not exam­ined for in this sample.
  • Just because the sought after fea­tures of the mod­i­fied Duquenois-Levine exam­i­na­tion were present in the sec­ond sam­ple doesn’t nec­es­sar­ily mean that the fea­tures sought after in the micro­scopic mor­pho­log­i­cal exam­i­na­tion are there (because it was not exam­ined) and the sought after fea­tured of the TLC exam­i­na­tion are there (because it was not exam­ined) in this sec­ond sample.
  • Just because the sought after fea­tures of the TLC exam­i­na­tion were present in the third sam­ple doesn’t nec­es­sar­ily mean that the fea­tures sought after in the micro­scopic mor­pho­log­i­cal exam­i­na­tion are there (because it was not exam­ined) and the sought after fea­tured of the mod­i­fied Duquenois-Levine exam­i­na­tion are there (because it was not exam­ined) in this third sample.

All pros­e­cu­tions for the unlaw­ful pos­ses­sion of mar­i­juana requires as an essen­tial ele­ment of the crime for the gov­ern­ment to advance proof that the unknown sub­mit­ted for test­ing is in fact mar­i­juana (con­tains THC). Can the gov­ern­ment actu­ally do that based upon its typ­i­cal test­ing method?

In this series of posts we are going to exam­ine this seemly sim­ple question:

  1. What is the goal and the pur­pose of test­ing of unknowns gen­er­ally? How do we best design a test for marijuana?
  2. How is most mar­i­juana test­ing con­ducted in the United States?
  3. What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?
  4. What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?
  5. What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?
  6. Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?
  7. Is there a bet­ter way to test for marijuana?

Part 5: What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?

Thin Layer Chro­matog­ra­phy test­ing

What is it?

Thin Layer Chro­matog­ra­phy (TLC) is a chro­mato­graphic tech­nique. It is a com­bi­na­tion of a col­ori­met­ric test and is mea­sured in terms of separation.

Mechan­i­cally how is it preformed?

TLC depends on the sta­tion­ary phase, often a glass plate coated with sil­ica (it must be prop­erly  des­ic­cated or dried) and the mobile phase which is com­prised of a sol­vent mix­ture made of toluene and diethy­lamine typ­i­cally. When placed on a prop­erly dried plate and using a prop­erly mixed and pre­pared sol­vent, the sol­vent (the mobile phase) will be wicked up by the sil­ica with cap­il­lary action and travel up the plate. A sam­ple from the unknown is selected. It is mashed up in some mechan­i­cal process and in some processes call for it to be dis­solved. It is placed in the des­ig­nated spot. A test is com­pared by per­form­ing the reac­tion of the plate of a known sam­ple from an adju­di­cated source. This com­par­i­son is made with the analyst’s eyes. There will be dif­fer­ent spots on the plate at dif­fer­ent posi­tions from the ori­gin with var­i­ous inten­si­ties of color. The height and the color change are visu­al­ized. The spots typ­i­cally need to be visu­al­ized with a chem­i­cal spray such as Fast Blue B Salt (50 mg in 20 ml of NaOH (0.1 N)) or par­tic­u­lar lighting.

How is the typ­i­cal crime lab­o­ra­tory ana­lyst trained to con­duct this?

Once again, the ana­lyst is not trained in the fun­da­men­tals of how or why this process works. If you handed them a pen and paper and ask them to dia­gram and explain with specifics the chro­mato­graphic process, they would likely be baf­fled. Also for­eign to them would be the specifics as to why the sol­vent used to elute up the plate has to be in a spe­cific ratio and not another. In essence, it is another sub­jec­tive test as it is based upon per­cep­tion of color by the ana­lyst and the per­cep­tion of this height devel­op­ing on the plate ver­sus the adju­di­cated known.

The ana­lysts are not aca­d­e­m­i­cally trained in the the­ory of this tech­nique of chro­matog­ra­phy, and are not taught about cross-reactivity and false pos­i­tives or other sources of errors.

Is this a ver­i­fi­able test?

Much like the mod­i­fied Duquenois-Levine test, TLC is poten­tially ver­i­fi­able. Dig­i­tal cam­eras exist. Pho­tographs can be taken. Heck, even video can be taken to show how it is con­ducted on a par­tic­u­lar sam­ple from the unknown and this com­par­i­son to the adju­di­cated known. How­ever, the mod­ern prac­tice is to not take a sin­gle pho­to­graph, to not take advan­tage of video tech­nol­ogy and pro­duce noth­ing ver­i­fi­able in court that the test was even con­ducted or that the analyst’s per­cep­tion of the change did hap­pen and was cor­rectly interpreted.

Again, there are no crime lab­o­ra­to­ries that I am aware of that use the ACE-V (Analy­sis, Com­par­i­son, Eval­u­a­tion, and Ver­i­fi­ca­tion) tech­nique that one would find in fin­ger­print iden­ti­fi­ca­tion with a dou­ble check in real time by a fel­low bench ana­lyst. In essence, it is checked one time, by one per­son with no dou­ble check by another, and noth­ing pro­duced that proves that the analy­sis was done or that the fea­tures that are reported as present were in fact objec­tively present.

Is there empir­i­cal valid­ity stud­ies that prove that this is a spe­cific and con­fir­ma­tory test?

Once again, this type of test­ing has not been proven to be a val­i­dated method to test specif­i­cally for THC. There are well-known and dis­cov­ered false pos­i­tives which includes cof­fee, basil and even tobacco products.

Once again, the same issues that are dis­cussed prove true with this test­ing. This TLC test is pre­formed on a totally dif­fer­ent sam­ple from the unknown that is not sub­jected to the micro­scopic mor­pho­log­i­cal exam­i­na­tion or the mod­i­fied Duquenois-Levine col­ori­met­ric test.

The Foren­sic Sci­ence Geek of the Week

Please visit the www.TheTruthAboutForensicScience.com Face­Book fan page.

Forensic Science Geek of the Week

The week 78 “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week” hon­ors goes to: Steven W. Her­nan­dez, Esquire

Accord­ing to our Geek, the fol­low­ing is offered:

Steven W. Her­nan­dez, Esq., is admit­ted to prac­tice in State of New Jer­sey and the United States Dis­trict Court for the Dis­trict of New Jer­sey. He spe­cial­izes in DWI defense.  Mr. Her­nan­dez is a mem­ber of the National Col­lege of DUI Defense (NCDD). Amer­i­can Bar Asso­ci­a­tion, The New Jer­sey Bar Asso­ci­a­tion, and the Ocean County Bar Asso­ci­a­tion.  He has suc­cess­fully com­pleted the DUI Detec­tion & Stan­dard­ized Field Sobri­ety Test­ing, the same course rec­og­nized by the National High­way Trans­porta­tion Safety Admin­is­tra­tion (NHTSA), used to train police per­son­nel. Mr. Her­nan­dez was the first attor­ney in New Jer­sey to receive his Foren­sic Sobri­ety Assess­ment (FSA) Cer­tifi­cate, by demon­strat­ing knowl­edge of the sci­ence and foren­sic use of road­side sobri­ety testing.

Con­grat­u­la­tions to our Foren­sic Sci­ence Geek of the Week winner!

OFFICIAL QUESTION:

Forensic Science Geek of the Week Challenge
Foren­sic Sci­ence Geek of the Week Challenge

1. What is pic­tured above?

2. What is it used for?

3. How does it work?

4. Are there any known issues with respect to its foren­sic suit­abil­ity? If so, what?

 

Our Geek of the Week answered:

(1) Duquenois-Levine Reagent is a test for detect­ing mar­i­juana and hashish and the residue of THC in para­pher­na­lia. It con­tains vanillin, con­cen­trated hydrochlo­ric acid, and chlo­ro­form (2) It used by : a. Remov­ing clip; b. Adding sub­stance into pouch c. Replace clip d. Break left ampoule, agi­tate for one minute. Look for any color that formed (none should occur). e. Break mid­dle ampoule, agi­tate until color changes. Look for any color formed (a rich violet-blue should occur). f. Break right ampoule, and agi­tate for 5 sec­onds. g. Observe color change: if slate grey upper level over pur­ple lower level, then it is pos­i­tive for mar­i­juana. (3) Foren­si­cally speak­ing it is not very reli­able. The test has been known to pro­duce false pos­i­tives and has been known iden­tify some plants, such as nut­meg as marijuana.

[BLOGGER’S NOTE: The myth of spe­cific iden­ti­fi­ca­tion of Mar­i­juana in crim­i­nal court Part 4: What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?

A very hon­or­able men­tion goes to: Anthaony Gra­viano who wrote:

1. The image is of an Duquenois-Levine reagent pouch which is part of an ODV Nar­coPouch nar­cotics field test­ing kit.

2. It is used by inves­ti­ga­tors to test an unknown sub­stance for the pres­ence of Marijuana.

3. The test is per­formed by first plac­ing approx­i­mately 10-20mg of the tar­get sub­stance into the ampoule that comes with the test­ing kit. The Duquenois-Levine reagent pouch is then added to the ampoule. The mix­ture is shaken to allow for a proper mix­ture and if the mix­ture turns pur­ple it is an indi­ca­tion that the unknown sub­stance is Marijuana.

4. This test has a num­ber of known issues. There is room for error in the tester’s per­cep­tion of the color. Fac­tors such as improper light­ing or the back­ground in which the test is viewed upon could impact the tester’s per­cep­tion and they might think the test turned pur­ple when it is, in-fact not. Start­ing in the 1960’s and 70’s, there were var­i­ous stud­ies that indi­cated the Duquenois-Levine reagent test is not spe­cific to Mar­i­juana. In 1969, a UK gov­ern­ment sci­en­tist named M.J. de Faubert Maun­der dis­cov­ered that 25 dif­fer­ent organic sub­stances pro­duced a sim­i­lar color as Mar­i­juana in the Duquenois-Levine reagent test, warn­ing that it should never be used as the only con­clu­sive evi­dence. Another study which was pub­lished in 1975 in The Jour­nal of Crim­i­nal Defense stated “The micro­scopic and chem­i­cal screen­ing tests presently used in mar­i­juana analy­sis are not spe­cific even in com­bi­na­tion for ‘mar­i­juana’ defined in any way.” In con­clu­sion, the Nar­coPouch field test­ing kit is not foren­si­cally suit­able to be used by itself as con­clu­sive evi­dence that an unknown sub­stance is Marijuana.

The Hall of Fame for the www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week:

Week 1: Chuck Ram­say, Esquire

Week 2: Rick McIn­doe, PhD

Week 3: Chris­tine Funk, Esquire

Week 4: Stephen Daniels

Week 5: Stephen Daniels

Week 6: Richard Mid­dle­brook, Esquire

Week 7: Chris­tine Funk, Esquire

Week 8: Ron Moore, B.S., J.D.

Week 9: Ron Moore, B.S., J.D.

Week 10: Kelly Case, Esquire and Michael Dye, Esquire

Week 11: Brian Man­ches­ter, Esquire

Week 12: Ron Moore, B.S., J.D.

Week 13: Ron Moore, B.S., J.D.

Week 14: Josh D, Lee, Esquire

Week 15: Joshua Dale, Esquire and Steven W. Her­nan­dez, Esquire

Week 16: Chris­tine Funk, Esquire

Week 17: Joshua Dale, Esquire

Week 18: Glen Nee­ley, Esquire

Week 19: Amanda Bynum, Esquire

Week 20: Josh D. Lee, Esquire

Week 21: Glen Nee­ley, Esquire

Week 22:  Stephen Daniels

Week 23:  Ron Moore, B.S., J.D.

Week 24: Bobby Spinks

Week 25:  Jon Woolsey, Esquire

Week 26: Mehul B. Anjaria

Week 27: Richard Mid­dle­brook, Esquire

Week 28:Ron Moore, Esquire

Week 29: Ron Moore, Esquire

Week 30: C. Jef­frey Sifers, Esquire

Week 31: Ron Moore, Esquire

Week 32: Mehul B. Anjaria

Week 33: Andy Johnston

Week 34: Ralph R. Ris­ten­batt, III

Week 35: Brian Man­ches­ter, Esquire

Week 36: Ron Moore, Esquire

Week 37: Jef­frey Benson

Week 38: Pam King, Esquire

Week 39: Josh D. Lee, Esquire

Week 40: Robert Lantz, Ph.D.

WEEK 41: UNCLAIMED, IT COULD BE YOU!

Week 42: Steven W. Her­nan­dez, Esquire

Week 43:Ron Moore, Esquire

Week 44: Mehul B. Anjaria

Week 45: Mehul B. Anjaria

Week 46:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 48: Leslie M. Sam­mis, Esquire

Week 49: Ron Moore, Esquire

Week 50: Jef­fery Benson

Week 51: Mehul B. Anjaria

Week 52: Ron Moore, Esquire

Week 53: Eric Ganci, Esquire

Week 54: Charles Sifers, Esquire and Tim Huey, Esquire

Week 55: Joshua Andor, Esquire

Week 56: Brian Man­ches­ter, Esquire

Week 57: Ron Moore, Esquire

Week 58: Eric Ganci, Esquire

Week 59: Ron Moore, Esquire

Week 60: Brian Man­ches­ter, Esquire

Week 61: William Her­ringer, Esquire

Week 62: UNCLAIMED IT COULD BE YOU!

Week 63: Gin­ger Moss

Week 64: Richard L. Hol­comb, Esquire

Week 65: John L. Buck­ley, Esquire

Week 66: Jeff Sifers, Esquire

Week 67: Josh D. Lee, Esquire

Week 68: Dr. Bar­bara Von­der­haar, PhD.

Week 69: Chris­tine Funk, Esquire

Week 70: Mehul B. Anjaria

Week 71: Ron Moore, Esquire

Week 72: Mehul B. Anjaria

Week 73: Josh D. Lee, Esquire

Week 74: Kim Kehe­ley Frye, Esquire

Week 75: Mehul B. Anjaria and Peter Carini, Esquire

Week 76: Kim Kehe­ley Frye, Esquire

Week 77: Mehul B. Anjaria

Week 78: Steven W. Her­nan­dez, Esquire

Week 79: UNCLAIMED. IT COULD BE YOU!

Week 8o: Justin Har­ris, Esquire

Week 81: UNCLAIMED. IT COULD BE YOU!

Week 82: Jay Tiftick­jian, Esquire

 

The Foren­sic Sci­ence Geek of the Week

Please visit the www.TheTruthAboutForensicScience.com Face­Book fan page.

Forensic Science Geek of the Week

The week 82 “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week” hon­ors goes to: Jay Tiftick­jian, Esquire

Accord­ing to our Geek, the fol­low­ing is offered:

Den­ver attor­ney Jay Tiftick­jian car­ries a Pre­em­i­nent AV® rat­ing in Martindale-Hubbell, the high­est rat­ing an attor­ney can achieve based on con­fi­den­tial rat­ings by mem­bers of the Col­orado Judi­ciary and Col­orado Bar. Martindale-Hubbell is the nation’s old­est and most respected legal direc­tory, and a Pre­em­i­nent AV® rat­ing is an out­stand­ing achieve­ment, demon­strat­ing an attorney’s superb legal abil­ity and pro­fes­sional ethics. A Pre­em­i­nent AV® rat­ing demon­strates an attor­ney has reached the height of pro­fes­sional excellence.

In 2010, Mr. Tiftick­jian was granted Life Mem­ber­ship sta­tus with the Col­orado Crim­i­nal Defense Bar based on his con­tri­bu­tions to crim­i­nal defense in Col­orado. There are cur­rently fewer than 20 Life Mem­bers of the CCDB, and attor­ney Tiftick­jian is proud to be the youngest Life Member.

Mr. Tiftick­jian is listed in the pres­ti­gious Bar Reg­is­ter of Pre­em­i­nent Lawyers™ for crim­i­nal defense. The Bar Reg­is­ter of Pre­em­i­nent Lawyers is a national direc­tory for only those prac­tic­ing attor­neys at the top of their field, and is the defin­i­tive guide of the most dis­tin­guished lawyers and law firms in the United States.

In 2010 and 2011, Mr. Tiftick­jian was nom­i­nated as a Super Lawyer: Ris­ing Star for recog­ni­tion of the up and com­ing defense attor­neys in Col­orado. This award rec­og­nizes the best crim­i­nal defense attor­neys in Col­orado under 40 as voted on by the Col­orado Bar. No more than 2.5 per­cent of attor­neys receive this award. Mr. Tiftick­jian was also pro­filed in 2010 and 2011’s 5280 Mag­a­zine for Den­ver DUI/DWI defense and crim­i­nal defense.

Mr. Tiftick­jian is a mem­ber of The National Asso­ci­a­tion of Crim­i­nal Defense Lawyers (NACDL), The National Col­lege of DUI Defense (NCDD), The Col­orado Bar Asso­ci­a­tion (CBA) and The Col­orado Crim­i­nal Defense Bar (CCDB). He has also received cer­ti­fi­ca­tion in the National High­way Traf­fic Safety Admin­is­tra­tion (NHSTA) DUI field sobri­ety test prac­ti­tioner and instruc­tor course.

Mr. Tiftick­jian reg­u­larly attends ses­sions within the National Col­lege for DUI Defense, includ­ing its yearly DUI train­ing con­ducted at Har­vard Law School. This is the NCDD’s sig­na­ture pro­gram to develop DUI trial skills, includ­ing inno­v­a­tive ways to attack breath tests, blood tests, field sobri­ety tests, and the prosecution’s police offi­cers and expert witnesses.

Con­grat­u­la­tions to our Foren­sic Sci­ence Geek of the Week winner!

OFFICIAL QUESTION:

Forensic Science Geek of the Week Challenge
Foren­sic Sci­ence Geek of the Week Challenge
1. What is com­puter pro­gram that is pic­tured above?

2. What type of search is is conducting?

3. Are there any known issues with respect to this type of search’s foren­sic suit­abil­ity? If so, what?

Our Geek of the Week answered:.

This is enCase and it looks to be doing a hard­drive search, although I am not cer­tain of this as I have never had my paws on the pro­gram. It is known to have prob­lems with search­ing emails.

[BLOGGER’S NOTE: This is a tough one in terms of ques­tion num­ber 2 and 3 for the very rea­son Attor­ney Tiftick­jian sug­gests. The defense bar and defense inves­ti­ga­tors are not sup­posed to have access to enCase. The pro­gram itself is pre­form­ing what is called a hash value search. The National Cen­ter for Miss­ing and Exploited Chil­dren (NCMEC) data­base of SHA-1 hash val­ues is a data­base library of SHA-1 hash val­ues of sup­pos­edly adju­di­cated child pornog­ra­phy images. What pre­cisely it con­tains and how it is deter­mined to be “adju­di­cated” is not totally avail­able to the pri­vate sec­tor, fur­ther increas­ing the dif­fi­culty of com­puter foren­sic exam­i­na­tions for the defen­dants. Specif­i­cally, the screen­shot fea­tured in our Geek of the Week chal­lenge shows the results of match­ing files against hash sets. The “Hash Set” col­umn shows what “pack­age” the file belongs to. The com­ments in the sig­na­ture col­umn tell you about inter­est­ing bits – for instance, logo.sys is a bitmap even though its exten­sion is .sys.]

The Hall of Fame for the www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week:

Week 1: Chuck Ram­say, Esquire

Week 2: Rick McIn­doe, PhD

Week 3: Chris­tine Funk, Esquire

Week 4: Stephen Daniels

Week 5: Stephen Daniels

Week 6: Richard Mid­dle­brook, Esquire

Week 7: Chris­tine Funk, Esquire

Week 8: Ron Moore, B.S., J.D.

Week 9: Ron Moore, B.S., J.D.

Week 10: Kelly Case, Esquire and Michael Dye, Esquire

Week 11: Brian Man­ches­ter, Esquire

Week 12: Ron Moore, B.S., J.D.

Week 13: Ron Moore, B.S., J.D.

Week 14: Josh D, Lee, Esquire

Week 15: Joshua Dale, Esquire and Steven W. Her­nan­dez, Esquire

Week 16: Chris­tine Funk, Esquire

Week 17: Joshua Dale, Esquire

Week 18: Glen Nee­ley, Esquire

Week 19: Amanda Bynum, Esquire

Week 20: Josh D. Lee, Esquire

Week 21: Glen Nee­ley, Esquire

Week 22:  Stephen Daniels

Week 23:  Ron Moore, B.S., J.D.

Week 24: Bobby Spinks

Week 25:  Jon Woolsey, Esquire

Week 26: Mehul B. Anjaria

Week 27: Richard Mid­dle­brook, Esquire

Week 28:Ron Moore, Esquire

Week 29: Ron Moore, Esquire

Week 30: C. Jef­frey Sifers, Esquire

Week 31: Ron Moore, Esquire

Week 32: Mehul B. Anjaria

Week 33: Andy Johnston

Week 34: Ralph R. Ris­ten­batt, III

Week 35: Brian Man­ches­ter, Esquire

Week 36: Ron Moore, Esquire

Week 37: Jef­frey Benson

Week 38: Pam King, Esquire

Week 39: Josh D. Lee, Esquire

Week 40: Robert Lantz, Ph.D.

WEEK 41: UNCLAIMED, IT COULD BE YOU!

Week 42: Steven W. Her­nan­dez, Esquire

Week 43:Ron Moore, Esquire

Week 44: Mehul B. Anjaria

Week 45: Mehul B. Anjaria

Week 46:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 48: Leslie M. Sam­mis, Esquire

Week 49: Ron Moore, Esquire

Week 50: Jef­fery Benson

Week 51: Mehul B. Anjaria

Week 52: Ron Moore, Esquire

Week 53: Eric Ganci, Esquire

Week 54: Charles Sifers, Esquire and Tim Huey, Esquire

Week 55: Joshua Andor, Esquire

Week 56: Brian Man­ches­ter, Esquire

Week 57: Ron Moore, Esquire

Week 58: Eric Ganci, Esquire

Week 59: Ron Moore, Esquire

Week 60: Brian Man­ches­ter, Esquire

Week 61: William Her­ringer, Esquire

Week 62: UNCLAIMED IT COULD BE YOU!

Week 63: Gin­ger Moss

Week 64: Richard L. Hol­comb, Esquire

Week 65: John L. Buck­ley, Esquire

Week 66: Jeff Sifers, Esquire

Week 67: Josh D. Lee, Esquire

Week 68: Dr. Bar­bara Von­der­haar, PhD.

Week 69: Chris­tine Funk, Esquire

Week 70: Mehul B. Anjaria

Week 71: Ron Moore, Esquire

Week 72: Mehul B. Anjaria

Week 73: Josh D. Lee, Esquire

Week 74: Kim Kehe­ley Frye, Esquire

Week 75: Mehul B. Anjaria and Peter Carini, Esquire

Week 76: Kim Kehe­ley Frye, Esquire

Week 77: Mehul B. Anjaria

Week 78: UNCLAIMED. IT COULD BE YOU!

Week 79: UNCLAIMED. IT COULD BE YOU!

Week 8o: Justin Har­ris, Esquire

Week 81: UNCLAIMED. IT COULD BE YOU!

Week 82: Jay Tiftick­jian, Esquire

 

Our good friend, Ron Moore, Esquire writes us “I actu­ally had a client research­ing lawyers who looked at the Truth About Foren­sic Sci­ence geek of the week posts and liked my answers. It made a dif­fer­ence in who he decided to hire. Thanks!” So, there is a lot of value in www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week Chal­lenge. Try it out today.

Foren­sic Sci­ence Geek of the Week

Forensic Science Geek of the Week
Foren­sic Sci­ence Geek of the Week

Thanks to the com­bined inspi­ra­tion of Chris­tine Funk, Esquire and Chuck Ram­say, Esquire, a new twist of this blog is being intro­duced. A weekly fun foren­sic sci­ence challenge/trivia ques­tion. The win­ner will be affec­tion­ately dubbed “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week.”

Rules:

  1. The chal­lenge will be posted Sun­day morn­ing 12 noon EST.
  2. Answers to the chal­lenge will be entered by respond­ing to this blog post or thewww.TheTruthAboutForensicScience.com Face­Book fan page.
  3. All com­ments that are answers to this blog will released after 9pm EST.
  4. The first com­plete and cor­rect answer will be awarded the envi­ous title of “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week”
  5. www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week” is enti­tled a one time post of his/her pic­ture on this blog and the www.TheTruthAboutForensicScience.com Face­Book fan page. The cov­eted title will be his/her for that week. Addi­tion­ally, a win­ner will be allowed one link to one web­page of his/her choice. Both the pic­ture and the weblink is sub­ject to the approval of Justin J McShane, Esquire and will only be screened for appro­pri­ate taste.
  6. The win­ner will be announced Sun­day night.
  7. A win­ner may only repeat two times in a row, then will have to sit out a week to be eli­gi­ble again. This per­son, who was the two time in a row win­ner, may answer the ques­tion, but will be dis­qual­i­fied from the honor so as to allow oth­ers to participate.
  8. This is for learn­ing and for fun. EVERYONE IS ENCOURAGED TO TRY TO ANSWER THE WEEKLY QUESTION. So give it a shot.

Here it is:

The www.TheTruthAboutForensicScience.com “Foren­sic Sci­ence Geek of the Week” chal­lenge ques­tion. Remem­ber the first full and com­plete answer wins the honor and also gets his/her photo dis­played, brag­ging rights for the week and finally web­site promotion.

OFFICIAL QUESTION:

Forensic Science Geek of the Week Challenge

Foren­sic Sci­ence Geek of the Week Challenge

1. What is com­puter pro­gram that is pic­tured above?

2. What type of search is is conducting?

3. Are there any known issues with respect to this type of search’s foren­sic suit­abil­ity? If so, what?

The Hall of Fame for the www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week:

Week 1: Chuck Ram­say, Esquire

Week 2: Rick McIn­doe, PhD

Week 3: Chris­tine Funk, Esquire

Week 4: Stephen Daniels

Week 5: Stephen Daniels

Week 6: Richard Mid­dle­brook, Esquire

Week 7: Chris­tine Funk, Esquire

Week 8: Ron Moore, B.S., J.D.

Week 9: Ron Moore, B.S., J.D.

Week 10: Kelly Case, Esquire and Michael Dye, Esquire

Week 11: Brian Man­ches­ter, Esquire

Week 12: Ron Moore, B.S., J.D.

Week 13: Ron Moore, B.S., J.D.

Week 14: Josh D, Lee, Esquire

Week 15: Joshua Dale, Esquire and Steven W. Her­nan­dez, Esquire

Week 16: Chris­tine Funk, Esquire

Week 17: Joshua Dale, Esquire

Week 18: Glen Nee­ley, Esquire

Week 19: Amanda Bynum, Esquire

Week 20: Josh D. Lee, Esquire

Week 21: Glen Nee­ley, Esquire

Week 22:  Stephen Daniels

Week 23:  Ron Moore, B.S., J.D.

Week 24: Bobby Spinks

Week 25:  Jon Woolsey, Esquire

Week 26: Mehul B. Anjaria

Week 27: Richard Mid­dle­brook, Esquire

Week 28:Ron Moore, Esquire

Week 29: Ron Moore, Esquire

Week 30: C. Jef­frey Sifers, Esquire

Week 31: Ron Moore, Esquire

Week 32: Mehul B. Anjaria

Week 33: Andy Johnston

Week 34: Ralph R. Ris­ten­batt, III

Week 35: Brian Man­ches­ter, Esquire

Week 36: Ron Moore, Esquire

Week 37: Jef­frey Benson

Week 38: Pam King, Esquire

Week 39: Josh D. Lee, Esquire

Week 40: Robert Lantz, Ph.D.

WEEK 41: UNCLAIMED, IT COULD BE YOU!

Week 42: Steven W. Her­nan­dez, Esquire

Week 43:Ron Moore, Esquire

Week 44: Mehul B. Anjaria

Week 45: Mehul B. Anjaria

Week 46:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 48: Leslie M. Sam­mis, Esquire

Week 49: Ron Moore, Esquire

Week 50: Jef­fery Benson

Week 51: Mehul B. Anjaria

Week 52: Ron Moore, Esquire

Week 53: Eric Ganci, Esquire

Week 54: Charles Sifers, Esquire and Tim Huey, Esquire

Week 55: Joshua Andor, Esquire

Week 56: Brian Man­ches­ter, Esquire

Week 57: Ron Moore, Esquire

Week 58: Eric Ganci, Esquire

Week 59: Ron Moore, Esquire

Week 60: Brian Man­ches­ter, Esquire

Week 61:William Her­ringer, Esquire

Week 62: UNCLAIMED IT COULD BE YOU!

Week 63: Gin­ger Moss

Week 64: Richard L. Hol­comb, Esquire

Week 65: John L. Buck­ley, Esquire

Week 66: Jeff Sifers, Esquire

Week 67: Josh D. Lee, Esquire

Week 68: Dr. Bar­bara Von­der­haar, PhD.

Week 69: Chris­tine Funk, Esquire

Week 70: Mehul B. Anjaria

Week 71: Ron Moore, Esquire

Week 72: Mehul B. Anjaria

Week 73: Josh D. Lee, Esquire

Week 74: Kim Kehe­ley Frye, Esquire

Week 75: Mehul B. Anjaria and Peter Carini, Esquire

Week 76: Kim Kehe­ley Frye, Esquire

Week 77: Mehul B. Anjaria

Week 78: UNCLAIMED. IT COULD BE YOU!

Week 79: UNCLAIMED. IT COULD BE YOU!

Week 8o: Justin Har­ris, Esquire

Week 81: UNCLAIMED. IT COULD BE YOU!

 

In this series of posts we are going to exam­ine this seemly sim­ple question:

  1. What is the goal and the pur­pose of test­ing of unknowns gen­er­ally? How do we best design a test for marijuana?
  2. How is most mar­i­juana test­ing con­ducted in the United States?
  3. What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?
  4. What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?
  5. What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?
  6. Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?
  7. Is there a bet­ter way to test for marijuana?

Part 4: What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?

Mod­i­fied Duquenois-Levine test­ing

What is it?

It is tech­ni­cally referred to as a col­ori­met­ric test. In short, it is a color test. A totally dif­fer­ent selec­tion of the unknown is sam­pled and sub­jected to this test. A reagent is added to the unknown. The reagent is made up of a com­bi­na­tion of vanillin, acetalde­hyde, and ethanol in a spe­cific ratio of these com­po­nent materials.

Mechan­i­cally how is it preformed?

This totally sep­a­rate sam­ple from the unknown is placed into typ­i­cally a test tube. A cer­tain amount of the Duquenois reagent is added (typ­i­cally about 10 drops). The tube is closed. The tube is agi­tated (shaken) for an unspec­i­fied period. The tube is reopened. Con­cen­trated hydrochlo­ric acid is then added (usu­ally about 20 drops). The tube is closed. It is agi­tated (shaken) again. Any color change is then noted. The tube is reopened. Chlo­ro­form is added. The tube is closed. It is again agi­tated (shaken) or vor­texed (mixed). The ana­lyst is look­ing for a color change (thought to be vio­let or pur­ple) and a sep­a­ra­tion into two layers.

This is the end result of the modified Duquenois-Levine test. This picture is after the HCl and the chloroform are added. There is a deep purple color at the top and then a pink color at the bottom.

This is the end result of the mod­i­fied Duquenois-Levine test. This pic­ture is after the HCl and the chlo­ro­form are added. There is a deep pur­ple color at the top and then a pink color at the bottom.

How is the typ­i­cal crime lab­o­ra­tory ana­lyst trained to con­duct this test?

They are trained in the process and the steps in the mechan­i­cal process. No crime lab­o­ra­tory ana­lyst is ever instructed by a doc­toral level ana­lyt­i­cal chemist as to why this process results in any sort of color change or the way the phys­i­cal sep­a­ra­tion occurs in the col­ors with the addi­tion of chlo­ro­form or why it these changes hap­pen at all.

In essence, it is a sub­jec­tive test as it is based upon per­cep­tion of color by the ana­lyst and the per­cep­tion of this devel­op­ing of a sep­a­rate layer once chlo­ro­form is added.

It is a sec­ond sam­ple that is tested. Prior to the test­ing by mod­i­fied Duquenois-Levine tech­nique, there is no micro­scopic mor­pho­log­i­cal exam­i­na­tion con­ducted on this sam­ple. There is no TLC analy­sis on this sample.

It is tested one time and then dis­carded. No other test­ing is per­formed on this sample.

The ana­lysts are not aca­d­e­m­i­cally trained in the the­ory of the reagent use, and are not taught about cross-reactivity and false pos­i­tives or other sources of errors.

Is this a ver­i­fi­able test?

Poten­tially, yes. Dig­i­tal cam­eras exist. Pho­tographs can be taken just like the one above. Heck, even video can be taken to show how it is con­ducted on a par­tic­u­lar sam­ple from the unknown. How­ever, the mod­ern prac­tice is to not take pho­tos. They video­tape noth­ing.  The lab­o­ra­tory pro­duces noth­ing ver­i­fi­able in court that the test was even con­ducted or that the analyst’s per­cep­tion of the change in color did hap­pen and was cor­rect or that there was this sep­a­ra­tion once the chlo­ro­form is added.

Again, there are no crime lab­o­ra­to­ries that I am aware of that use the ACE-V (Analy­sis, Com­par­i­son, Eval­u­a­tion, and Ver­i­fi­ca­tion) tech­nique that one would find in fin­ger­print iden­ti­fi­ca­tion with a dou­ble check in real time by a fel­low bench ana­lyst. In essence, it is checked one time, by one per­son with no dou­ble check by another, and noth­ing pro­duced that proves that the analy­sis was done or that the fea­tures that are reported as present were in fact objec­tively present.

Is there empir­i­cal valid­ity stud­ies that prove that this is a spe­cific and con­fir­ma­tory test?

Nope. In fact the empir­i­cal stud­ies clearly show the oppo­site. It is not spe­cific for THC. The reac­tion is not unique to THC. In fact, the stud­ies clearly show that it is not even mean­ing­fully selec­tive for THC. Chi­nese moth­er­wort if tested, by this method will turn vio­let. But that is not all. There are a great many plants that yield sim­i­lar color results when Duquenois-Levine test­ing is applied. Yet ana­lysts are taught that is color change is diag­nos­tic of THC. Naka­mura him­self pub­lished and acknowl­edges that M. J. de Faubert Maun­der listed 25 species of plants which exhib­ited vio­let to pur­ple col­ors in the Duqenois test, and were extractable in chlo­ro­form, which is the dis­tin­guish­ing fea­tures of the Duquenois Levine test. (502) Naka­mura tested 23 of those species noted by M. J. de Faubert Maun­der. Using the Duquenois-Leine test, he found that the vio­let to pur­ple color when test­ing the leaves of the fol­low­ing (502):

  • cof­fee
  • a species of gum copal called Caplafer conjugata
  • gum Kawri
  • wood sage
  • Thuja occi­den­talis
  • San­darac

He pub­lished that he tested and found vio­let to pur­ple color reac­tions using the Duquenois-Levine test in other plants as well, and not just the leafy por­tions of these plants. (502). That list includes:

  • cala­mus
  • cul­ver root
  • gin­ger
  • gum animi
  • gum copal
  • gum myrrh
  • henna
  • let­tuce opium
  • san­dal wood
  • tolu
  • wood betony
  • liquorice
  • nut­meg
  • poi­son flag

The United Nations study on mar­i­juana con­cludes that Duquenois-Levine test on sub­stances com­pletely unre­lated to mar­i­juana will give false pos­i­tives too. This includes Arthemisia Gran­cunulus, Euca­lyp­tus Glab­u­lus Labill and sev­eral other herbs that are very com­mon such as rose­mary, thyme, and sage.

None of these items above con­tain THC. Yet, they fea­ture these color changes and some even have the sep­a­ra­tion when chlo­ro­form is added that the ana­lysts are trained to look for when apply­ing these reagents.

While we have exam­ined the pro­pri­ety and suit­abil­ity of this mod­i­fied Duquenois-Levine test­ing in the iden­ti­fi­ca­tion of THC, we can­not judge this Duquenois-Levine test in iso­la­tion. We must remem­ber and in fair­ness this is a 3 test process and tech­nique. What com­pounds the issue of the pos­si­bil­ity of error is that as the ana­lyst is not using the same sam­ple through­out each of these 3 tests. It is in fact, three dif­fer­ent sam­ples are taken of the unknown and each test gets its own sam­ple with no sam­ple receiv­ing the ben­e­fit of all three tests. The ana­lyst pre­sumes that the sam­pling and the sam­ple selec­tion of the unknown is homo­ge­neous mean­ing that each sam­ple will be exactly iden­ti­cal. This is not a jus­ti­fied sci­en­tific assump­tion (An assump­tion is not drawn from evi­dence; it is a hypoth­e­sis {my assump­tion can be tested by look­ing at the dic­tio­nary}. A pre­sump­tion implies a basis in evi­dence {the legal pre­sump­tion of inno­cence}) Just because the sought after fea­tures of the micro­scopic mor­pho­log­i­cal exam­i­na­tion were present in the first sam­ple, that doesn’t nec­es­sar­ily mean that it will be present in the next sam­ple that the ana­lyst now sub­jects to mod­i­fied Duquenois-Levine testing.

Naka­mura tested and found that mace and nut­meg, which yielded col­ors sim­i­lar to that obtained with mar­i­juana with the Duquenois-Levine test, could be “cred­i­bly con­fused” with mar­i­juana based on micro­scopic appear­ance. (502)

In this series of posts we are going to exam­ine these seemly sim­ple questions:

  1. What is the goal and the pur­pose of test­ing of unknowns gen­er­ally? How do we best design a test for marijuana?
  2. How is most mar­i­juana test­ing con­ducted in the United States?
  3. What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?
  4. What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?
  5. What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?
  6. Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?
  7. Is there a bet­ter way to test for marijuana?

Part 3: What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?

Micro­scopic mor­pho­log­i­cal examination

What is it?

The micro­scopic mor­pho­log­i­cal exam­i­na­tion in short is an exer­cise of botan­i­cal iden­ti­fi­ca­tion using a microscope.

Mechan­i­cally how is it preformed?

A very small amount of the dried unknown is selected. This becomes the sam­ple. The sam­ple is placed on a micro­scopic slide. A drop or two of water is then added to the slide. The slides are exam­ined at vary­ing lev­els of mag­ni­fi­ca­tion and under dif­fer­ent light con­di­tions. What the ana­lyst is look­ing for is two dis­tinct mor­pho­log­i­cal fea­tures. They are look­ing for micro­scopic “hairs” on the unknown. These are cys­tolithic hairs and glan­du­lar hairs. Cys­tolithic hairs are often likened to like lit­tle bear claws in their appearance.

photomicrographs of cystolithic hairs

pho­tomi­cro­graphs of cys­tolithic hairs

The sec­ond type of hair is called a glan­du­lar hair. These are fre­quently remarked as look­ing like mushrooms.

photomicrographs of glandular hairs

pho­tomi­cro­graphs of glan­du­lar hairs

Some tech­niques call for the use of hydrochlo­ric acid after they look for these hairs. A few drops of HCL are added by the ana­lyst. The ana­lyst then looks to see if there is some unspec­i­fied effer­ves­cence under the light of the microscope.

How is the typ­i­cal crime lab­o­ra­tory ana­lyst trained to con­duct this form of testing?

The ques­tion becomes what expe­ri­ence level in botany and tax­on­omy and microscopy does the ana­lyst truly have? Very few under­grad­u­ate pro­grams exist in botany in the United States. Most ana­lysts have on the job train­ing where another per­son who like­wise have no for­mal train­ing in botany or tax­on­omy that instructs them. It also involves the use of a micro­scope. For­mal train­ing in microscopy is required in order to use a micro­scope prop­erly and to prop­erly inter­pret what the human eye sees through var­i­ous pow­ers and light­ing con­di­tions of the micro­scope. At the end of the in-house train­ing, the typ­i­cal ana­lyst can­not typ­i­cally express the fam­ily, the genus and the species that is “mar­i­juana” or at what power and under what light­ing con­di­tions they saw the mor­pho­log­i­cal characteristics.

This sam­ple that is exam­ined under the micro­scope is then dis­carded. All future or addi­tional test­ing is con­ducted on totally dif­fer­ent sam­ples from the unknown.

Is this a ver­i­fi­able test?

It poten­tially is. There is a device that can be linked to the micro­scope to take pic­tures of what the ana­lyst thinks he or she sees. This is called a pho­tomi­cro­graph. In fact the pic­tures above come from just such a micro­scope that is equipped with one. A dig­i­tal cam­era attached to a micro­scope is very com­monly used in sci­ence. They are very mod­er­ately priced. As they are dig­i­tal cam­eras, the cost of pro­duc­tion and print­ing and data stor­age is neg­li­gi­ble. It is fre­quently used in other types of com­par­a­tive exam­i­na­tions such as some higher lev­els of foren­sic firearm or tool­mark iden­ti­fi­ca­tion. I know of no lab­o­ra­tory in the United States that relies upon micro­scopic mor­pho­log­i­cal exam­i­na­tion that uses mod­ern tech­nol­ogy and pro­duces pho­tomi­cro­graphs. In fact, few, if any, crime lab­o­ra­to­ries use the ACE-V (Analy­sis, Com­par­i­son, Eval­u­a­tion, and Ver­i­fi­ca­tion) tech­nique that one would find in fin­ger­print iden­ti­fi­ca­tion using a stereo-microscope and a dou­ble check in real time by a fel­low bench ana­lyst. In essence, the unknown is checked one time, by one per­son with no dou­ble check by another, and noth­ing is pro­duced that proves that the analy­sis was done or that the fea­tures that are reported as present were in fact objec­tively present. There is no proof.

Is there empir­i­cal valid­ity stud­ies that prove that this is a spe­cific and con­fir­ma­tory test?

No. There are no empir­i­cal and robust val­i­da­tion stud­ies that con­clude that this form of micro­scopic mor­pho­log­i­cal exam­i­na­tion even when the two botan­i­cal fea­tures (cys­tolithic and glan­du­lar hairs) are objec­tively present yield a valid opin­ion that the plant exam­ined is def­i­nitely con­tains THC. There are no stud­ies that say the two fea­tures means that there is THC present. In fact, what stud­ies that are out there con­clude that this form of mor­pho­log­i­cal exam­i­na­tion using a micro­scope is per­haps not even selec­tive. In the orig­i­nal stud­ies by Naka­mura, he indi­cated that cys­toliths of var­i­ous types are found in the leaves of a num­ber of dicots. (497). He also indi­cated that the pres­ence of cys­toliths is not diag­nos­tic for a fam­ily, let alone a genus of plants. (497) Naka­mura specif­i­cally noted that cys­toliths are found on a great num­ber of plants includ­ing but not lim­ited to: hops plants (500), oregano (500), lemon thyme (501), sil­ver thyme (501), and rose­mary (501). Naka­mua he specif­i­cally noted 63 “rep­re­sen­ta­tive” species in 13 plant gen­era that con­tain cys­toliths in table 5 of his arti­cle (501) Naku­mura indi­cated that he made NO attempt to pre­pare a com­pre­hen­sive list­ing of species bear­ing cys­tolith hairs sim­i­lar to those found in cannabis “because of the sheer enor­mity of the task to exam­ine 31,874+ dicotyle­dons.” (500). For instance, in one genus found in Table 5 of his arti­cle, the Loasa, he specif­i­cally noted 9 species that had cys­toliths; how­ever, he went on to say that there were actu­ally some 80 species of that genus known to have sim­i­lar hairs. (501). He fully acknowl­edged that his list­ing was not com­pre­hen­sive. So it is accu­rate and very fair to say that the 63 “rep­re­sen­ta­tive” species that have cys­toliths that were noted by Naka­mura in Table 5 of his arti­cle are not an exhaus­tive list. Other stud­ies agree that at least 6 other sub­stances also have hairs that con­tain these two fea­tures (cys­tolithic and glan­du­lar hairs). In terms of the addi­tional step of adding HCL to the sam­ple and exam­in­ing for effer­ves­cence under the light of the micro­scope, it is quite clear that other sub­stances can pro­duce the same effer­ves­cence when a few drops of hydrochlo­ric acid are added to them. For exam­ple net­tles and cat­nip do exactly that.

Some folks main­tain and tes­tify under oath every day in the United States that this unver­i­fied micro­scopic mor­pho­log­i­cal exam­i­na­tion is diag­nos­tic of iden­ti­fi­ca­tion of THC pres­ence in an unknown. There is no sci­en­tific sup­port for this type of testimony.

One thing that every ana­lyst should agree with is that sim­ply because these hairs are present and if they con­duct HCL addi­tion and if there is effer­ves­cence that does not mean that the unknown con­tains THC. This is why they have to do addi­tional test­ing, mean­ing the mod­i­fied Duquenois-Levine and Thin Layer Chro­matog­ra­phy testing.

What is fre­quently not part of any mor­pho­log­i­cal exam­i­na­tion for cannabis is what botanists have noted to be other fea­tures con­sis­tent with cannabis. The sim­pli­fied exam­i­na­tion for the typ­i­cal foren­sic sci­ence iden­ti­fi­ca­tion is pur­pose­fully designed to make this exam­i­na­tion and con­clu­sions from it eas­ier to per­form by non-botanists. As with every­thing in life, the more cri­te­rion attached to qual­ify some­thing the least likely there will be a qual­i­fi­ca­tion. The exam­i­na­tion of cannabis and espe­cially a mor­pho­log­i­cal exam­i­na­tion by untrained botanists should not be made easy. All of the fea­tures that are known to be diag­nos­tic by the world of botany should be used not sim­ply the easy ones. For exam­ple, botanists have noted that that cannabis has ses­sile glands as well as con­tain­ing ser­rated edges of the leaves and com­pound palmate struc­ture mean­ing sev­eral leaflets arise from the same point. The addi­tion of all of the known mor­pho­log­i­cal fea­tures known to true botanists as diag­nos­tic of cannabis would make this exam­i­na­tion more robust and the result more selec­tive than the sim­plis­tic exam­i­na­tion that now per­me­ates the foren­sic sci­ence world.

Our good friend, Ron Moore, Esquire writes us “I actu­ally had a client research­ing lawyers who looked at the Truth About Foren­sic Sci­ence geek of the week posts and liked my answers. It made a dif­fer­ence in who he decided to hire. Thanks!” So, there is a lot of value in www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week Chal­lenge. Try it out today.

Foren­sic Sci­ence Geek of the Week

Forensic Science Geek of the Week
Foren­sic Sci­ence Geek of the Week

Thanks to the com­bined inspi­ra­tion of Chris­tine Funk, Esquire and Chuck Ram­say, Esquire, a new twist of this blog is being intro­duced. A weekly fun foren­sic sci­ence challenge/trivia ques­tion. The win­ner will be affec­tion­ately dubbed “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week.”

Rules:

  1. The chal­lenge will be posted Sun­day morn­ing 12 noon EST.
  2. Answers to the chal­lenge will be entered by respond­ing to this blog post or thewww.TheTruthAboutForensicScience.com Face­Book fan page.
  3. All com­ments that are answers to this blog will released after 9pm EST.
  4. The first com­plete and cor­rect answer will be awarded the envi­ous title of “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week”
  5. www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week” is enti­tled a one time post of his/her pic­ture on this blog and the www.TheTruthAboutForensicScience.com Face­Book fan page. The cov­eted title will be his/her for that week. Addi­tion­ally, a win­ner will be allowed one link to one web­page of his/her choice. Both the pic­ture and the weblink is sub­ject to the approval of Justin J McShane, Esquire and will only be screened for appro­pri­ate taste.
  6. The win­ner will be announced Sun­day night.
  7. A win­ner may only repeat two times in a row, then will have to sit out a week to be eli­gi­ble again. This per­son, who was the two time in a row win­ner, may answer the ques­tion, but will be dis­qual­i­fied from the honor so as to allow oth­ers to participate.
  8. This is for learn­ing and for fun. EVERYONE IS ENCOURAGED TO TRY TO ANSWER THE WEEKLY QUESTION. So give it a shot.

Here it is:

The www.TheTruthAboutForensicScience.com “Foren­sic Sci­ence Geek of the Week” chal­lenge ques­tion. Remem­ber the first full and com­plete answer wins the honor and also gets his/her photo dis­played, brag­ging rights for the week and finally web­site promotion.

OFFICIAL QUESTION:

Forensic Science Geek of the Week Challenge

Foren­sic Sci­ence Geek of the Week Challenge

1. What is pic­tured above?

2. What tech­nol­ogy is it based upon?

3. Are there any known issues with respect to its foren­sic suit­abil­ity? If so, what?

The Hall of Fame for the www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week:

Week 1: Chuck Ram­say, Esquire

Week 2: Rick McIn­doe, PhD

Week 3: Chris­tine Funk, Esquire

Week 4: Stephen Daniels

Week 5: Stephen Daniels

Week 6: Richard Mid­dle­brook, Esquire

Week 7: Chris­tine Funk, Esquire

Week 8: Ron Moore, B.S., J.D.

Week 9: Ron Moore, B.S., J.D.

Week 10: Kelly Case, Esquire and Michael Dye, Esquire

Week 11: Brian Man­ches­ter, Esquire

Week 12: Ron Moore, B.S., J.D.

Week 13: Ron Moore, B.S., J.D.

Week 14: Josh D, Lee, Esquire

Week 15: Joshua Dale, Esquire and Steven W. Her­nan­dez, Esquire

Week 16: Chris­tine Funk, Esquire

Week 17: Joshua Dale, Esquire

Week 18: Glen Nee­ley, Esquire

Week 19: Amanda Bynum, Esquire

Week 20: Josh D. Lee, Esquire

Week 21: Glen Nee­ley, Esquire

Week 22:  Stephen Daniels

Week 23:  Ron Moore, B.S., J.D.

Week 24: Bobby Spinks

Week 25:  Jon Woolsey, Esquire

Week 26: Mehul B. Anjaria

Week 27: Richard Mid­dle­brook, Esquire

Week 28:Ron Moore, Esquire

Week 29: Ron Moore, Esquire

Week 30: C. Jef­frey Sifers, Esquire

Week 31: Ron Moore, Esquire

Week 32: Mehul B. Anjaria

Week 33: Andy Johnston

Week 34: Ralph R. Ris­ten­batt, III

Week 35: Brian Man­ches­ter, Esquire

Week 36: Ron Moore, Esquire

Week 37: Jef­frey Benson

Week 38: Pam King, Esquire

Week 39: Josh D. Lee, Esquire

Week 40: Robert Lantz, Ph.D.

WEEK 41: UNCLAIMED, IT COULD BE YOU!

Week 42: Steven W. Her­nan­dez, Esquire

Week 43:Ron Moore, Esquire

Week 44: Mehul B. Anjaria

Week 45: Mehul B. Anjaria

Week 46:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 48: Leslie M. Sam­mis, Esquire

Week 49: Ron Moore, Esquire

Week 50: Jef­fery Benson

Week 51: Mehul B. Anjaria

Week 52: Ron Moore, Esquire

Week 53: Eric Ganci, Esquire

Week 54: Charles Sifers, Esquire and Tim Huey, Esquire

Week 55: Joshua Andor, Esquire

Week 56: Brian Man­ches­ter, Esquire

Week 57: Ron Moore, Esquire

Week 58: Eric Ganci, Esquire

Week 59: Ron Moore, Esquire

Week 60: Brian Man­ches­ter, Esquire

Week 61:William Her­ringer, Esquire

Week 62: UNCLAIMED IT COULD BE YOU!

Week 63: Gin­ger Moss

Week 64: Richard L. Hol­comb, Esquire

Week 65: John L. Buck­ley, Esquire

Week 66: Jeff Sifers, Esquire

Week 67: Josh D. Lee, Esquire

Week 68: Dr. Bar­bara Von­der­haar, PhD.

Week 69: Chris­tine Funk, Esquire

Week 70: Mehul B. Anjaria

Week 71: Ron Moore, Esquire

Week 72: Mehul B. Anjaria

Week 73: Josh D. Lee, Esquire

Week 74: Kim Kehe­ley Frye, Esquire

Week 75: Mehul B. Anjaria and Peter Carini, Esquire

Week 76: Kim Kehe­ley Frye, Esquire

Week 77: Mehul B. Anjaria

Week 78: UNCLAIMED. IT COULD BE YOU!

Week 79: UNCLAIMED. IT COULD BE YOU!

Week 8o: Justin Har­ris, Esquire

 

The Foren­sic Sci­ence Geek of the Week

Please visit the www.TheTruthAboutForensicScience.com Face­Book fan page.

Forensic Science Geek of the Week

The week 80 “www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week” hon­ors goes to: Justin Har­ris, Esquire

Justin Harris, Esquire

Justin Har­ris, Esquire

Accord­ing to our Geek, the fol­low­ing is offered:

Justin Har­ris joined Greco & Asso­ciates, P.C. in Sep­tem­ber, 2011. He is a native Hous­ton­ian, born and raised in North­west Hous­ton, before his fam­ily relo­cated to Mag­no­lia, Texas in 1997. Justin takes pride in his hard work, atten­tion to detail, pro­fes­sion­al­ism and work­ing very hard to fight for his clients in each and every case.

Justin began work­ing in the legal field as a project assis­tant for one of the top law firms in the coun­try in 2000. Over the next decade, Justin worked on thou­sands of cases. Through­out col­lege and law school, he worked for var­i­ous law firms in both Austin and Hous­ton, each spe­cial­iz­ing in dif­fer­ent areas of the law. After his grad­u­a­tion from South­west­ern Uni­ver­sity in George­town, Texas, Justin took sev­eral years to gain legal expe­ri­ence before he embarked upon his life­long dream of attend­ing law school. He enrolled at South Texas Col­lege of Law in 2008 and ful­filled his dream in 2011 when he grad­u­ated and suc­cess­fully passed the Texas bar exam, gain­ing admit­tance to the State Bar of Texas. Dur­ing law school, Justin clerked for sev­eral promi­nent Hous­ton crim­i­nal defense attor­neys, work­ing in Har­ris, Fort Bend, Mont­gomery, Galve­ston, Bra­zo­ria, Grimes, Jef­fer­son and Lib­erty Coun­ties. At each, he gained invalu­able expe­ri­ence, being men­tored by trial and appel­late attor­neys as well as for­mer pros­e­cu­tors. Upon grad­u­a­tion from law school, he received offers to join dif­fer­ent law firms, but set­tled on Greco & Asso­ciates, P.C. based upon their rep­u­ta­tion for fight­ing hard for their clients’ cases and after see­ing their ded­i­ca­tion to each client. After three years of work­ing while attend­ing law school, Justin has worked closely on hun­dreds of crim­i­nal cases and par­tic­i­pated in over a dozen crim­i­nal trials.

Justin joins Greco & Asso­ciates, P.C. with nearly twelve years of legal expe­ri­ence at dif­fer­ent posi­tions within var­i­ous law firms. In addi­tion to his love of the law, Justin greatly enjoys play­ing the gui­tar, attend­ing live music events, trav­el­ing and spend­ing time with his fam­ily and friends.

Con­grat­u­la­tions to our Foren­sic Sci­ence Geek of the Week winner!

OFFICIAL QUESTION:

Forensic Science Geek of the Week Challenge
Foren­sic Sci­ence Geek of the Week Challenge

1. What is pic­tured above?

2. What is it used for?

3. Are there any known issues with respect to its foren­sic suit­abil­ity? If so, what?

Our Geek of the Week answered:

Here’s my best guess:

1. A dis­tance mea­sur­ing wheel, some­times also called a “rolling dis­tance mea­sur­ing wheel.”

2. It is rolled along a sur­face (usu­ally the ground) from one point to another point to pro­vide a dis­tance that is mea­sured by a small odometer-type gauge (typ­i­cally).  It is most often appro­pri­ate for mea­sur­ing dis­tances when a typ­i­cal tape mea­sure would not work. The known cir­cum­fer­ence of the wheel is used to pro­vide a lin­ear mea­sure­ment through the “odometer”.

3. Sev­eral issues with its reli­a­bil­ity: First, if the dis­tance between point A to point B involves bumps, dips or inun­da­tions in the sur­face, the dis­tance pro­vided by the wheel will be affected and inac­cu­rate.  Sec­ond, depend­ing on the qual­ity of the par­tic­u­lar device, the “odome­ter” may not be as accu­rate as another.  Third, and most sig­nif­i­cantly, a lot of the wheel’s accu­racy is depen­dent upon the user–if the user does not keep close, firm con­tact with the sur­face the wheel rolls over, it may allow the wheel to spin with­out being in con­tact with the sur­face; this will also dis­tort the mea­sure­ment pro­vided by the wheel.  Finally, and, admit­tedly, I am not com­pletely sure of this one, the devices are typ­i­cally unable to be cal­i­brated.  After pro­longed use, the cir­cum­fer­ence of the wheel may dimin­ish (if even slight, it will become more pro­nounced over longer dis­tances).  The gears in the “odome­ter” may slacken, etc.

[BLOGGER’S NOTE: Very hon­or­able men­tion goes to Ron Moore, Esquire who answered: “This is a Roll-a-tape. Its used in larger crime scenes and espe­cially vehic­u­lar acci­dents to make dis­tance mea­sure­ments for doc­u­ment­ing the posi­tion of evi­dence. Its not the most accu­rate device for mea­sur­ing dis­tances, but then again, mil­lime­ter accu­racy usu­ally isn’t required. there are more mod­ern meth­ods avail­able that use dGSP and lasers for mea­sur­ing. I’ve never heard of a roll-a-tape being cal­i­brated, nor have I heard of evi­dence being excluded because the roll-a-tape might have been a lit­tle off.”]

The Hall of Fame for the www.TheTruthAboutForensicScience.com Foren­sic Sci­ence Geek of the Week:

Week 1: Chuck Ram­say, Esquire

Week 2: Rick McIn­doe, PhD

Week 3: Chris­tine Funk, Esquire

Week 4: Stephen Daniels

Week 5: Stephen Daniels

Week 6: Richard Mid­dle­brook, Esquire

Week 7: Chris­tine Funk, Esquire

Week 8: Ron Moore, B.S., J.D.

Week 9: Ron Moore, B.S., J.D.

Week 10: Kelly Case, Esquire and Michael Dye, Esquire

Week 11: Brian Man­ches­ter, Esquire

Week 12: Ron Moore, B.S., J.D.

Week 13: Ron Moore, B.S., J.D.

Week 14: Josh D, Lee, Esquire

Week 15: Joshua Dale, Esquire and Steven W. Her­nan­dez, Esquire

Week 16: Chris­tine Funk, Esquire

Week 17: Joshua Dale, Esquire

Week 18: Glen Nee­ley, Esquire

Week 19: Amanda Bynum, Esquire

Week 20: Josh D. Lee, Esquire

Week 21: Glen Nee­ley, Esquire

Week 22:  Stephen Daniels

Week 23:  Ron Moore, B.S., J.D.

Week 24: Bobby Spinks

Week 25:  Jon Woolsey, Esquire

Week 26: Mehul B. Anjaria

Week 27: Richard Mid­dle­brook, Esquire

Week 28:Ron Moore, Esquire

Week 29: Ron Moore, Esquire

Week 30: C. Jef­frey Sifers, Esquire

Week 31: Ron Moore, Esquire

Week 32: Mehul B. Anjaria

Week 33: Andy Johnston

Week 34: Ralph R. Ris­ten­batt, III

Week 35: Brian Man­ches­ter, Esquire

Week 36: Ron Moore, Esquire

Week 37: Jef­frey Benson

Week 38: Pam King, Esquire

Week 39: Josh D. Lee, Esquire

Week 40: Robert Lantz, Ph.D.

WEEK 41: UNCLAIMED, IT COULD BE YOU!

Week 42: Steven W. Her­nan­dez, Esquire

Week 43:Ron Moore, Esquire

Week 44: Mehul B. Anjaria

Week 45: Mehul B. Anjaria

Week 46:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 47:Ron Moore, Esquire

Week 48: Leslie M. Sam­mis, Esquire

Week 49: Ron Moore, Esquire

Week 50: Jef­fery Benson

Week 51: Mehul B. Anjaria

Week 52: Ron Moore, Esquire

Week 53: Eric Ganci, Esquire

Week 54: Charles Sifers, Esquire and Tim Huey, Esquire

Week 55: Joshua Andor, Esquire

Week 56: Brian Man­ches­ter, Esquire

Week 57: Ron Moore, Esquire

Week 58: Eric Ganci, Esquire

Week 59: Ron Moore, Esquire

Week 60: Brian Man­ches­ter, Esquire

Week 61: William Her­ringer, Esquire

Week 62: UNCLAIMED IT COULD BE YOU!

Week 63: Gin­ger Moss

Week 64: Richard L. Hol­comb, Esquire

Week 65: John L. Buck­ley, Esquire

Week 66: Jeff Sifers, Esquire

Week 67: Josh D. Lee, Esquire

Week 68: Dr. Bar­bara Von­der­haar, PhD.

Week 69: Chris­tine Funk, Esquire

Week 70: Mehul B. Anjaria

Week 71: Ron Moore, Esquire

Week 72: Mehul B. Anjaria

Week 73: Josh D. Lee, Esquire

Week 74: Kim Kehe­ley Frye, Esquire

Week 75: Mehul B. Anjaria and Peter Carini, Esquire

Week 76: Kim Kehe­ley Frye, Esquire

Week 77: Mehul B. Anjaria

Week 78: UNCLAIMED. IT COULD BE YOU!

Week 79: UNCLAIMED. IT COULD BE YOU!

Week 8o: Justin Har­ris, Esquire

 

In 2006, more than 829,000 peo­ple were arrested in this coun­try for marijuana-related offenses alone. Since 1937 with the pas­sage and adop­tion of the Mar­i­huana Tax Act, mar­i­juana has been effec­tively pro­hib­ited in the United States.

Lit­er­ally mil­lions upon mil­lions of peo­ple have been accused of pos­sess­ing mar­i­juana. The ques­tion becomes are these con­vic­tions sci­en­tif­i­cally supported?

In this series of posts we are going to exam­ine this seemly sim­ple question:

  1. What is the goal and the pur­pose of test­ing of unknowns gen­er­ally? How do we best design a test for marijuana?
  2. How is most mar­i­juana test­ing con­ducted in the United States?
  3. What is micro­scopic mor­pho­log­i­cal exam­i­na­tion? Is it a “good” test?
  4. What is the mod­i­fied Duquenois-Levine test? Is it a “good” test?
  5. What is Thin Layer Chro­matog­ra­phy? Is it a “good” test?
  6. Is the com­bi­na­tion of all three tests cre­ate a “good” test­ing scheme?
  7. Is there a bet­ter way to test for marijuana?

Part 2: How is most mar­i­juana test­ing con­ducted in the United States?

So what hap­pens in Amer­ica, here, now and today in the iden­ti­fi­ca­tion of marijuana?

  • Are the tests pre­sump­tive or confirmatory?
  • Are the meth­ods used verifiable?
  • Does it result in a spe­cific or selec­tive identification?

Let’s take a look at how mar­i­juana is tested in the United States today in the super-majority of cases. It all begins with a police offi­cer or another per­son who seizes an unknown sub­stance. It’s green. It’s veg­etable like. It looks like mar­i­juana to them. Per­haps they do some sort of quick and dirty test on the side of the road using a fast col­ori­met­ric test and there is a change in color. This is a true unknown. We don’t know what it is. We can­not con­clude what it is based upon sim­ply look­ing at it with our own two eyes or even by our sense of smell. This is why we have foren­sic sci­en­tists and fur­ther test­ing because those types of obser­va­tions may lead to an improper or incor­rect result.

attempts at identifying marijuana at the gross or macro level can be misleading

Attempts at iden­ti­fy­ing mar­i­juana at the gross or macro level can be misleading

A basic fun­da­men­tal ques­tion becomes: What makes mar­i­juana ille­gal to pos­sess? What makes mar­i­juana ille­gal is that it con­tains the phar­ma­co­dy­nam­i­cally sub­stance known as Delta-9 Tetrahy­dro­cannabi­nol (THC).

Given that THC is what makes mar­i­juana ille­gal, then log­i­cally what would be best is if we were design tests that react exclu­sively to THC and noth­ing else and pro­duces ver­i­fi­able data that iden­ti­fies that the green veg­etable sub­stance in fact specif­i­cally con­tains THC.

Because we can’t tell what it is just by look­ing at it or just by smelling it, then we must sub­ject this unknown to test­ing. Largely in the United States there is a régime or process of how crime lab­o­ra­to­ries con­duct this test­ing. The triad of test­ing that is con­ducted are:

  1. Micro­scopic mor­pho­log­i­cal examination
  2. Mod­i­fied Duquenois-Levine testing
  3. Thin Layer Chromatography

This is gen­er­ally referred to as the Thorton-Nakumura protocol.

In order to answer our ques­tions of speci­ficity, ver­i­fi­ca­tion, and con­fir­ma­tory we need to look at each of these three test­ing tech­niques. In our next blog posts over the next sev­eral weeks each of these tests will be scrutinized.